Callus Culture Pharmacognosy II & IV Year

Meaning of Callus Culture:

Callus is formed by the proliferation of the parent tissue. The cells of a callus are parenchymatous, amorphous and unorganised. Generally callus is formed as a result of injury at the cut ends of a stem or a root. Localised centres of activity is reĀ­corded in a callus.

When tissues on culture produce unorganised mass of callus with no regular form then it is called callus culture. Callus formation from isolated stem segments of Populous was first observed by Rechinger in 1893. Working with cambial tissues of carrot and tobacco first prolonged callus culture were simultaneously reported by Gautheret in Paris, Nobecourt in England and White in Princeton, USA in 1930.

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